Journal: Neuroscience

Article Title: Connexin and AMPA Receptor Expression Changes Over Time in the Rat Olfactory Bulb

doi: 10.1016/j.neuroscience.2012.06.070

Figure Lengend Snippet: Statistical results for measured mRNA

Article Snippet: Primary antisera used in this study were purchased from Cell Signaling Technology (CST; Danvers, MA, USA), Invitrogen, Millipore (Billerica, MA, USA), and Sigma–Aldrich and are as follows, with the epitope, dilution/buffer, and catalog number in parentheses: Invitrogen rabbit anti-Cx36 (cytoplasmic loop between second and third transmembrane domains; 1:250 in 4% milk/TBST; 51-6200), Invitrogen rabbit anti-Cx36 (C-terminus; 1:250 in 1% milk/TBST; 51-6300), Invitrogen mouse anti-Cx36 (C-terminus; 1:500 in 4% milk/TBST; 37-4600), Invitrogen rabbit anti-Cx43 (C-terminus; 1:500 in OBBT; 71-0700), Invitrogen rabbit anti-Cx45 (C-terminus; 1:250/OBBT; 40-7000), Millipore mouse anti-GluR1 (N-terminus; 1:500/OBBT; MAB2263), CST rabbit anti-GluR2 (N-terminus; 1:500/OBBT; 5306S), CST rabbit anti-GluR3 (residues surrounding Pro590 of human GluR3; 1:1500/OBBT; 4676S), CST rabbit anti-GluR4 (residues surrounding Gln890 of human GluR4; 1:1000 in 5% bovine serum albumin/TBST*; 8070S), CST mouse anti-β-III-tubulin (C-terminus; 1:2000/OBBT; 4466S), and Sigma–Aldrich rabbit anti-β-III-tubulin (residues 442–446; 1:5000 in 4% milk/TBST; SAB4300623).

Techniques:

Journal: Neuroscience

Article Title: Connexin and AMPA Receptor Expression Changes Over Time in the Rat Olfactory Bulb

doi: 10.1016/j.neuroscience.2012.06.070

Figure Lengend Snippet: Changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) subunit mRNA across dark and light phases in rat OB. Line plots of (A) GluR1, (B) GluR2, (C) GluR3, and (D) GluR4 mRNA expression over 48-h. Sample size and notation as in Fig. 1. GluR1, GluR2, and GluR3 mRNA changes were statistically significant across the 48-h (Kruskal–Wallis H test, P < 0.05) but only GluR1 was rhythmic for both 24-h periods (harmonic regression analysis, P < 0.05). GluR1 and GluR4 were rhythmic according to JTK_CYCLE analysis (P < 0.05).

Article Snippet: Primary antisera used in this study were purchased from Cell Signaling Technology (CST; Danvers, MA, USA), Invitrogen, Millipore (Billerica, MA, USA), and Sigma–Aldrich and are as follows, with the epitope, dilution/buffer, and catalog number in parentheses: Invitrogen rabbit anti-Cx36 (cytoplasmic loop between second and third transmembrane domains; 1:250 in 4% milk/TBST; 51-6200), Invitrogen rabbit anti-Cx36 (C-terminus; 1:250 in 1% milk/TBST; 51-6300), Invitrogen mouse anti-Cx36 (C-terminus; 1:500 in 4% milk/TBST; 37-4600), Invitrogen rabbit anti-Cx43 (C-terminus; 1:500 in OBBT; 71-0700), Invitrogen rabbit anti-Cx45 (C-terminus; 1:250/OBBT; 40-7000), Millipore mouse anti-GluR1 (N-terminus; 1:500/OBBT; MAB2263), CST rabbit anti-GluR2 (N-terminus; 1:500/OBBT; 5306S), CST rabbit anti-GluR3 (residues surrounding Pro590 of human GluR3; 1:1500/OBBT; 4676S), CST rabbit anti-GluR4 (residues surrounding Gln890 of human GluR4; 1:1000 in 5% bovine serum albumin/TBST*; 8070S), CST mouse anti-β-III-tubulin (C-terminus; 1:2000/OBBT; 4466S), and Sigma–Aldrich rabbit anti-β-III-tubulin (residues 442–446; 1:5000 in 4% milk/TBST; SAB4300623).

Techniques: Expressing

Journal: Neuroscience

Article Title: Connexin and AMPA Receptor Expression Changes Over Time in the Rat Olfactory Bulb

doi: 10.1016/j.neuroscience.2012.06.070

Figure Lengend Snippet: Changes in AMPAR subunit protein expressed in the rat OB membrane across light and dark phases in the rat OB. (A) Anti-GluR3 (1:1500) and anti-beta-III-tubulin (1:2000) and (B) anti-GluR4 (1:1000) using experimental design, analysis, notation, sample size, and statistical metric as in Fig. 4. Nitrocellulose blots probed with anti-GluR4 were stripped and reprobed with anti-β-III-tubulin (1:2000). Arrows indicate expected size in kilodalton (Mr = 100 kDa for GluR3 and GluR4, and 50 kDa for tubulin). GluR3 and GluR4 protein changes were statistically significant across the 48-h (Kruskal–Wallis H test, P < 0.05) but not rhythmic across either 24-h tested (harmonic regression analysis, P > 0.05). GluR3 protein expression was statistically significant for JTK_CYCLE analysis (P < 0.05).

Article Snippet: Primary antisera used in this study were purchased from Cell Signaling Technology (CST; Danvers, MA, USA), Invitrogen, Millipore (Billerica, MA, USA), and Sigma–Aldrich and are as follows, with the epitope, dilution/buffer, and catalog number in parentheses: Invitrogen rabbit anti-Cx36 (cytoplasmic loop between second and third transmembrane domains; 1:250 in 4% milk/TBST; 51-6200), Invitrogen rabbit anti-Cx36 (C-terminus; 1:250 in 1% milk/TBST; 51-6300), Invitrogen mouse anti-Cx36 (C-terminus; 1:500 in 4% milk/TBST; 37-4600), Invitrogen rabbit anti-Cx43 (C-terminus; 1:500 in OBBT; 71-0700), Invitrogen rabbit anti-Cx45 (C-terminus; 1:250/OBBT; 40-7000), Millipore mouse anti-GluR1 (N-terminus; 1:500/OBBT; MAB2263), CST rabbit anti-GluR2 (N-terminus; 1:500/OBBT; 5306S), CST rabbit anti-GluR3 (residues surrounding Pro590 of human GluR3; 1:1500/OBBT; 4676S), CST rabbit anti-GluR4 (residues surrounding Gln890 of human GluR4; 1:1000 in 5% bovine serum albumin/TBST*; 8070S), CST mouse anti-β-III-tubulin (C-terminus; 1:2000/OBBT; 4466S), and Sigma–Aldrich rabbit anti-β-III-tubulin (residues 442–446; 1:5000 in 4% milk/TBST; SAB4300623).

Techniques: Expressing

Journal: Neuroscience

Article Title: Connexin and AMPA Receptor Expression Changes Over Time in the Rat Olfactory Bulb

doi: 10.1016/j.neuroscience.2012.06.070

Figure Lengend Snippet: Statistical results for measured membrane/organelle protein

Article Snippet: Primary antisera used in this study were purchased from Cell Signaling Technology (CST; Danvers, MA, USA), Invitrogen, Millipore (Billerica, MA, USA), and Sigma–Aldrich and are as follows, with the epitope, dilution/buffer, and catalog number in parentheses: Invitrogen rabbit anti-Cx36 (cytoplasmic loop between second and third transmembrane domains; 1:250 in 4% milk/TBST; 51-6200), Invitrogen rabbit anti-Cx36 (C-terminus; 1:250 in 1% milk/TBST; 51-6300), Invitrogen mouse anti-Cx36 (C-terminus; 1:500 in 4% milk/TBST; 37-4600), Invitrogen rabbit anti-Cx43 (C-terminus; 1:500 in OBBT; 71-0700), Invitrogen rabbit anti-Cx45 (C-terminus; 1:250/OBBT; 40-7000), Millipore mouse anti-GluR1 (N-terminus; 1:500/OBBT; MAB2263), CST rabbit anti-GluR2 (N-terminus; 1:500/OBBT; 5306S), CST rabbit anti-GluR3 (residues surrounding Pro590 of human GluR3; 1:1500/OBBT; 4676S), CST rabbit anti-GluR4 (residues surrounding Gln890 of human GluR4; 1:1000 in 5% bovine serum albumin/TBST*; 8070S), CST mouse anti-β-III-tubulin (C-terminus; 1:2000/OBBT; 4466S), and Sigma–Aldrich rabbit anti-β-III-tubulin (residues 442–446; 1:5000 in 4% milk/TBST; SAB4300623).

Techniques: